The treatment of burn has been progressed great deal by mordernized fluid therapy and use of
antibiotics.
The mortality of patients from the burn, however, has not been diminished despite of the modern
therapy. This has made many researchers to believe in existence of "burn toxin" and numerous papers
concerning the burn toxin has been published in the past several decades. Through reviewing the papers
it is suggested that there may be certain relation between the time following burn and the substances
which are released from the burned skin.
In order to clarify the relationship as well as the existence of toxic substances and it´s amount
following burn, the animal experiments were carried out.
Diffusates were collected from subcutaneous air baloon of the back of rats after making 3˚burn of
the ballooned skin at 30 min, 1 hour, 2 hours, 4 hours, 12 hours, 24 hours, 48 hours and 72 hours
intervals following burns. The amouont of protein in each diffusates was measured. Each diffusate was
then injected to the mouse intraperitoneally and the reaction of the mouse was carefully observed.
Results:
1. Average values of protein(s) in diffusates obtained form subcutaneous air ballon of burned skin of
rats were 1.21±0.069㎎/㎖ at 30min., 0.8±0.16㎎/㎖ at 1 hour, 1.04±0.01㎎/㎖ at 2
hours, 0.87±0.11㎎/㎖ at 4 hours, 1.0±0.02㎎/㎖ at 6 hours, 0.85±0.03㎎/㎖ at 12 hours,
2.39±0.08㎎/㎖ at 24 hours. 2.92±0.07㎎/㎖ at 48 hours, and 4.06±0.37㎎/㎖ at 72 hours.
2. All diffusates obtained form the burned skin of rats were lethal to the mice with it´s toxic
manifestation being from limitaion of motion to the death of animal.
3. The toxicity of the diffusates obtained at 1 hour, 2 hours, 4 hours following burn was most violent
and lethal to the mouse.
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